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| Study on Effects of Temperature on Metabolism of Aspergillus Flavus Based on Untargeted Metabolomics |
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| DOI： |
| KeyWord:aspergillus flavus metabolomics high-resolution mass spectrum aflatoxins biosynthesis temperature |
| Author | Institution |
| XIE Hua-li,LI Pei-wu,WANG Xiu-pin,ZHANG Qi,ZHANG Liang-xiao,WANG Tong，ZHANG Wen,WANG Xue-fang |
1.中国农业科学院油料作物研究所;2.农业部油料作物生物学与遗传育种重点实验室;3.农业部生物毒素检测重点实验室;4.农业部油料产品质量安全风险评估实验室;5.农业部油料及制品质量监督检验测试中心 |
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| Abstract: |
| A method of non-targeted metabolomics coupled to ultra-high performance liquid chromatography-high resolution mass spectrometry was used to investigate effect of temperature on the metabolism of aspergillus flavus. Multivariate statistical analysis on the metabolomics data was carried out by using chemometric methods of OrthoPLS-DA(alternative partial least squares discriminant analysis) etc. The metabolism characteristics of aspergillus flavus were identified by MS/MS and library searching. In the study,the non-targeted metabolomics analytical method was validated by using internal standard and pooled quality control samples. The method was applied to investigate the effect of temperature on the metabolome of aspergillus flavus,and 3593(T-test,p<0.01) differential metabolites were found under different temperature conditions. Twenty candidate biomarkers were screened out. The results showed that temperature significantly affected the biosynthetic pathways of tricarboxylic acid cycle,fatty acid,phenylalanine,tryptophan and tyrosine, and regulated the secondary metabolites biosynthetic pathway enzymesactivity of aflatoxin,cyclopiazonic acid and kojic acid. This study provided a new insight for risk assessment and early warning research of aflatoxin in China. |
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