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| Determination of Three Tetracyclines in Pig Kidney by High Performance Liquid Chromatography with Ionic Liquid Dispersive Liquid-Liquid Microextraction |
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| DOI： |
| KeyWord:ionic liquid(IL) dispersive liquid-liquid microextraction(DLLME) high performance liquid chromatography(HPLC) tetracycline antibiotics(TCs) pig kidney |
| Author | Institution |
| ZHANG Ying,DING Jian-hua,XIANG Hong-lin,LIU Peng-fei |
东华理工大学化学生物与材料科学学院 |
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| Abstract: |
| A method was established for the determination of three tetracycline antibiotics(TCs) residues such as oxytetracycline,tetracycline and chlortetracycline in pig kidney by high performance liquid chromatography(HPLC) coupled with dispersive liquid-liquid microextraction(DLLME).The influences of dispersant type,amount of ionic liquid,amount of dispersant,pH of sample solution,extraction time and effect of salts on extraction efficiency were investigated.The optimum experimental conditions were as follows:using acetone as the dispersant,a 50 μL ionic liquid(［BMIM］ PF6),a 140 μL dispersant,a pH 3.0 sample solution,a 15 min of extraction time and no salt.The results showed that this method provided a wide linearity in the range of 0.1-10.0 mg/L with correlation coefficients no less than 0.999 5.The relative standard deviations(RSDs) were 2.2%-3.1%for oxytetracycline,tetracycline and chlortetracycline.The detection limits(LOD) were in the range of 54-93 μg/L with the enrichment folds of 7.0-27.8,and the recoveries were 99.5%-101.1%.It was found that the accuracy and precision could meet the requirements for the rapid,green,sensitive and accurate detection of oxytetracycline,tetracycline and chlortetracycline residues in pig kidney. |
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