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| Simultaneous Screening and Quantitation of 31 Carbamate Pesticide Residues in Feed by Ultra Performance Liquid Chromatography-Orbitrap Mass Spectrometry |
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| DOI： |
| KeyWord:carbamate pesticides ultra performance liquid chromatography orbitrap mass spectrometry qualitation and quantitation feed |
| Author | Institution |
| WU Jian-ping,ZHOU Yue-rong,ZHANG Jing,LI Dan-ni,YAN Feng,PAN Juan |
上海市兽药饲料检测所 |
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| Abstract: |
| To screen and quantify 31 carbamate pesticide residues in feed simultaneously,an ultra performance liquid chromatography-orbitrap mass spectrometric method and the target compounds data base were established.The feed sample was extracted with 100 mol/L HCl-methanol(1∶3).The extract was high-speedily centrifuged,purified with acid,then diluted and injected for analysis.Thermo Syncronis C18 column(150 mm×2.1 mm,3 μm)was chosen as the solid phase with 0.1%formic acid and 0.1%formic acid acetonitrile as liquid phase by gradient elution.The mass spectrometer was operated in positive scan mode.One pair of parent ion and daughter ion were chosen for qualitation,and the response of the parent ion was used to achieve the quantitation.Under the optimal conditions,31 carbamates were separated well.Good linearities were obtained in their respective quantitative ranges with correlation coefficients(r2) more than 0.98.The average recoveries were in the range of 50.6%-109%and the relative standard deviations(RSDs) were not more than 8.9%.The method was convenient,economical,sensitive and accurate,and was applicable for the qualitation and quantitation of 31 carbamate pesticide residues in feed. |
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