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| Analysis of Thiamethoxam,Clothianidin and Spirotetramat and Their Metabolites Residues in Tomato Using QuEChERS Method with Ultra Performance Liquid Chromatography-Mass Spectrometry |
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| DOI： |
| KeyWord:QuEChERS UPLC-MS/MS pesticide residues tomato |
| Author | Institution |
| LIU Yan,OUYANG Di-qing,YE Yu-feng,WANG Cheng-qiu,JIAO Bi-ning,
ZHAO Qi-yang,ZHANG Yao-hai,AO Yan,WANG Hai-yan |
1.农业部柑桔产品质量安全风险评估实验室重庆，中国农业科学院柑桔研究所/西南大学柑桔研究所;2.西南大学食品科学学院;3.农业部柑桔及苗木质量监督检验测试中心;4.国家柑桔工程技术研究中心;5.柑桔学重庆市重点实验室;6.重庆市畜牧科学院 |
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| Abstract: |
| In this study,an analytical method was established for the determination of thiamethoxam and its metabolites clothianidin,spirotetramat and its four metabolites such as BYI08330-enol-glucoside,BYI08330-ketohydroxy,BYI08330-enol and BYI08330-mono-hydroxy residues in tomatoes using QuEChERS cleanup with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The residues in samples were extracted with acetonitrile,dehydrated with anhydrous magnesium sulfate and sodium chloride,then purified with primary secondary amine (PSA) sorbent and C18,finally detected by ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) under the multi-reactive ion monitoring mode(MRM) and quantified by external standard method.The results showed that good linearities for the seven analytes i.e.thiamethoxam,clothianidin,spirotetramat,BYI08330-enol-glucoside,BYI08330-mono-hydroxy,BYI08330-enol and BYI08330-ketohydroxy were observed in the concentration range of 0.2-2 000 μg/L with the correlation coefficients(r2) not less than 0.999 2. The limits of quantification(LOQs) were 0.002 mg/kg.The average recoveries at the fortified levels of 0.002,0.02,0.20 and 2.00 mg/kg were in the range of 79.9%-104% with the relative standard deviations of 0.94%-6.4%.With the advantages of rapidness,simplicity,high sensitivity and good reproducibility,the method is suitable for the determination of thiamethoxam,clothianidin,spirotetramat and their four metabolites residues in tomatoes. |
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