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| Highly Sensitive Detection of Ochratoxin A Based on FRET from Upconversion Nanoparticles to Gold Nanoparticles |
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| DOI： |
| KeyWord:upconversion nanoparticles gold nanoparticles fluorescence resonance energy transfer ochratoxin A aptamer |
| Author | Institution |
| ZHANG Ying-ying,QIAN Zhi-juan,XIE Zheng-jun,PENG Chi-fang |
1.江南大学食品科学与技术国家重点实验室;2.扬州出入境检验检疫局;3.江南大学食品学院 |
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| Abstract: |
| Upconversion nanoparticles were synthesized and modified with OTA aptamer,and gold nanoparticles were modified with complementary DNA strand(cDNA).Based these nanoparticles,a FRET aptasensor for OTA was successfully fabricated by using upconversion nanoparticles as energy donor and gold nanoparticles as energy acceptor .In the optimum conditions,this aptasensor obtained a detection limit of 0.001 ng/mL with a linear range of 0.001-10 ng/mL.The above method was applied in the detection of OTA in beer samples.At spiked levels of 0.01,0.1,1.0 ng/mL,the recoveries for OTA ranged from 100% to 119% with the relative standard deviations(RSDs) of 4.3%-4.9%.This method also demonstrated its advantages such as high sensitivity,good specificity,simplicity and low cost. |
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