Preparation of Porcine Liver Esterase Hybrid “Nanoflowers” and Their Hydrolyzing Properties toward Pyrethroid Pesticides
  
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KeyWord:hybrid nanoflowers  porcine liver esterase(PLE)  pyrethroid pesticide  enzyme activity  enzymolysis
  
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彭开敏,叶泰,曹慧,袁敏,于劲松,徐斐 上海理工大学医疗器械与食品学院,上海食品微生物工程研究中心
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Abstract:
      In this review,the porcine liver esterase(PLE) organic-inorganic hybrid nanoflowers(Ca3(PO4)2-PLE) were prepared in phosphate buffer by biomineralization strategy.The morphology and components of the prepared organic-inorganic hybrid nanoflowers were analyzed by scanning electron microscopy(SEM),energy dispersive spectrometry(EDS) and Fourier transform infrared spectroscopy(FT-IR).The parameters for the preparation of PLE hybrid nanoflowers were optimized.Results showed that the prepared PLE hybrid nanoflowers had the highest enzyme activity in phosphate buffer solution(pH 74) of 10 mmol/L,PLE concentration of 03 g/L and calcium ion concentration of 500 mmol/L at preparation temperature of 20 ℃,and its enzyme activity was 169% of the free enzyme in the same enzyme concentration.Furthermore,the PLE hybrid nanoflowers were used to study the enzymatic hydrolysis properties toward four pyrethroid pesticides.It was found that under the same conditions,the enzymatic hydrolysis rates of PLE hybrid nanoflowers were better than that of the free enzyme.In addition,the prepared PLE hybrid nanoflowers still remained over 70% of the enzymatic activity after twice uses of the pyrethroid pesticide enzymatic cycle.
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