Determination of 19 Triazole Fungicides in Chrysanthemum morifolium by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Selective Accelerated Solvent Extraction
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KeyWord:selective accelerated solvent extraction  ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)  triazole fungicide  Chrysanthemum morifolium
吴慧珍,李晓丹,汪建妹,王向军,杨华,徐杰,吉小凤,钱鸣蓉 1.浙江树人大学生物与环境工程学院;2.浙江农林大学林业与生物技术学院 ;3.浙江省农业科学院农产品质量标准研究所
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      A selective accelerated solvent extraction method combined with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 19 triazole fungicides in Chrysanthemum morifolium.The sample was extracted with acetonitrile,and the mixed dispersive sorbents(PSA,GCB and C18) were dispersed in the extract to reduce the effects of interfering components during the extraction procedure.After concentration,the extract was separated on a BEH C18 column using 2 mmol/L ammonium formate containing 0.1% formic acid and methanol as mobile phases,and detected under multi-reaction monitoring mode in electrospray positive ion mode.Matrix matched method was used for quantitative analysis.There were good linear relationships for 19 triazole fungicides in the range of 1.0-100.0 μg/L with their correlation coefficients(r2) not less than 0.99.The limits of detection and quantitation were 0.6-3.0 μg/kg and 2.1-10.0 μg/kg,respectively.Under the optimal conditions,the average recoveries for those compounds at three spiked levels of 10,50 and 100 μg/kg ranged from 70.3% to 95.3%,with relative standard deviations(RSD) of 0.60%-12%.This method is simple,reproducible and sensitive,and is applicable for the simultaneous determination of triazole fungicides in Chrysanthemum morifolium samples.