Comparison of Volatile Components in Hippophae Rhamnoides L.Extracted by Headspace Solid Phase Microextraction and Steam Distillation
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KeyWord:Hippophae rhamnoides L.  headspace solidphase microextraction(HS-SPME)  steam distillation(SD)  volatile components  gas chromatography-mass spectrometry(GC-MS)
张鹏云,李蓉,卢俊文,张朋杰,陈丽斯,张峰 1.中山出入境检验检疫局检验检疫技术中心;2.广东药科大学公共卫生学院;3.中国检验检疫科学研究院食品安全研究所
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      The conditions for extracting volatile components in Hippophae rhamnoides headspace solidphase microextraction(HS-SPME) were optimized.Gas chromatography-mass spectrometry(GC-MS) was used to detect the extracts by HS-SPME and steam distillation(SD).Results showed that the optimum conditions for HS-SPME were as follows:the extraction temperature and the extraction time were 70 ℃ and 50 min,respectively,and the desorption time and the equilibrium time were 7 min and 20 min,respectively.Under this condition,76 components were identified from the HS-SPME extracts,accounting for 90.19% of the total,and the main components were esters,aldehydes and ketones,respectively,while 56 components were identified from the SD extracts,accounting for 91.98% of the total,and the main components were esters.20 common components were detected in both the extracts.There are great differences in the types and contents of volatile oil in Hippophae rhamnoides L.extracted by the two extraction methods.The HS-SPME is more suitable for the rapid detection of volatile components in Hippophae rhamnoides L.