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| Scientific PapersStudy on PCR Droplet Detection Chip Based on Contactless Conductivity Detection |
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| DOI： |
| KeyWord:digital PCR droplets label-free contactless conductivity detection |
| Author | Institution |
| LIAO Cheng-yue,PENG Huo,GAO Ze-hang,QI Tong,ZHOU Hong-bo,ZHAO Jian-long |
1.State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Science;2.University of Chinese Academy of Sciences;3.ShanghaiTech University |
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| Abstract: |
| Digital PCR is a newly developed absolute quantitative analysis technique after real-time quantitative PCR,which is widely used in early detection and diagnosis of diseases and microbial detection.However,in the detection of digital PCR microreactors,conventional optical detection methods require complex optical equipments while the application environment is restricted and the cost is high.Therefore,a kind of PCR droplet detection chip based on contactless conductivity detection was developed by replacing the traditional optical detection with conductivity detection,which could effectively reduce the cost of the digital PCR result readout system.By optimizing the electrode structure and arrangement,a 3D electrode structure composed of three parallel electrodes is adopted to reduce the relative error and improve the sensitivity,and the detection on ratio of negative to positive PCR reaction droplets is achieved.The detecting throughput of the chip reaches up to 100 pcs per second,with a good relativity(r2=0.998) in the DNA template concentration range of 0.5×104-3×104 copies/μL. |
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