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| Construction of a Sensor for Gene Detection Based on G quadruplex-Hemin and Chain Amplification Reaction |
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| DOI： |
| KeyWord:DNA biosensor G-quadruplex hemin gene detection |
| Author | Institution |
| SUN Yan-xiu，SUN Xiao-fan |
1.Department of Basic Courses,Shenyang Institute of Technology;2.Wuxi Yaoming Biotechnology Co.,Ltd. |
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| Abstract: |
| In this study,a DNA biosensor was constructed for the sensitive detection of target DNA by chain reaction,with a specially designed DNA sequence as capture probe and a G-quadruplex-hemin complex as signal molecule.In the presence of target DNA,the capture DNA and the target DNA could recognize each other,while the target DNA could have a continuous chain reaction with the assistant probe,leading to the formation of a large number of G-quadruplex on the electrode surface.In the presence of hemin,the G-quadruplex combines with the hemin to form a G-quadruplex-hemin complex,which emits a strong electrochemical signal.Therefore, the detection of target DNA could be realized as the electrochemical signal obtained by differential pulse voltammetry(DPV) has a corresponding relationship with concentration of the target DNA in the system.Under the optimal conditions for the concentration of each component,the DPV current response has a good linear relationship with the target DNA concentration in the range of 0.01-10 pmol/L,and the minimum detection limit reaches to 8 fmol/L.With the advantages of high sensitivity and good specificity,the sensor has a good application prospect. |
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