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| Metabolomics Analysis of Lonicera japonica in Different Flowering Stages Based on Liquid Chromatography-Mass Spectrometry |
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| DOI： |
| KeyWord:Lonicera japonica metabolomics liquid chromatography-mass spectrometry(LC-MS) primary metabolites secondary metabolites flowering stages |
| Author | Institution |
| LI Li-li,LI Yue,LU Heng,WANG Xiao |
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Analysis and Test Center,Qilu University of TechnologyShandong Academy of Sciences |
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| Abstract: |
| An analytical method for the metabolomics of Lonicera japonica was established based on liquid chromatography-mass spectrometry(LC-MS).The extraction solution was optimized as a mixture solution of methanol and water(3∶1,by volume).The repeatability and precision of the method were investigated.It was found that the method could meet the requirements for metabolomics analysis with good stability.The primary and secondary metabolites in Lonicera japonica were identified by accurate mass,retention time and MS/MS data.A total of 157 metabolites were identified,including amino acids,nucleosides,fatty acids,lipids,phenolic acids,flavonoids and iridoid glycosides,etc.According to the metabolomics analysis of Lonicera japonica in different flowering stages,it was found that the secondary metabolites of phenolic acid,flavonoids and iridoid glycosides decreased significantly with the process of flowering stages.For the primary metabolites,the contents of most amino acids,nucleosides and oxidized fatty acids decreased significantly,while the contents of most Lyso PC,Lyso PE and fatty acids increased significantly.The results obtained provide theoretical guidances for study on the metabolic mechanism of medicinal components and the late planting and harvesting of Lonicera japonica. |
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