|
| Sensitive Detection of Bisphenol A Using an Immunosensor Based on Nano-gold/Poly-L-lysine Modified Screen-printed Electrode |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:nano-gold poly-L-lysine screen printed carbon electrode(SPCE) immunosensor bisphenol A(BPA) |
| Author | Institution |
| HE Zu-yu,LI Pu-wang,ZHOU Chuang,WANG Chao,Lü Ming-zhe,SONG Shu-hui,LIU Yun-hao,YANG Zi-ming |
1.Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences South Subtropical Crop Research Institute,Chinese Academy of Tropical Agricultural Sciences;2.Agricultural Products Processing Research Institute,Chinese Academy of Tropical Agricultural Sciences |
|
| Hits: 1705 |
| Download times: 696 |
| Abstract: |
| In this paper,an electrochemical immunosensor for the detection of bisphenol A(BPA) was constructed,based on the preparation of a poly-L-lysine/nano-gold modified electrode by electrodeposition,with disposable screen printed carbon electrode as the substrate.Results showed that,when the dilution factors for the antigen and the primary antibody were 1 000 times,the incubation time for the primary antibody was 60 min,and the pH value for the electrochemical detection environment was 7.4,there existed a good linear relationship between reduction peak current and BPA in the concentration range of 1-250 ng/mL,with a correlation coefficient(r2) of 0.993 and a detection limit of 0.85 ng/mL.With good specificity,reproducibility and stability,this method was applied in the detection of BPA in samples with spiked recoveries of 983%-104%,indicating that the BPA immunosensor has an application potential in the detection of bisphenol A in actual water samples. |
| Close |
|
|
|