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| Scientific PapersInstant Monitoring of Metabolomic Profile for Trace of Whole Blood Using Conductive Polymer Spray Ionization Mass Spectrometry |
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| DOI： |
| KeyWord:conductive polymer spray ionization(CPSI) point of care test metabolomic profile prognostic marker hepatectomy |
| Author | Institution |
| LI Chao,SONG Xiao-wei |
1.Department of Pharmaceutical Science,Beijing Hospital;National Center of Gerontology;Institute of Geriatric Medicine,Chinese Academy of Medical Science;Beijing Key Laboratory of Assessment of Clinical Drugs Risk and Individual ApplicationBeijing Hospital;2.Department of Chemistry,Fudan University |
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| Abstract: |
| The blood's metabolism could be used to provide a precise evaluation on the post surgery physiological status,which poses an urgent technical demand for a point of care test for global metabolomic profiling.Therefore,the practical value of conductive polymer spray ionization mass spectrometry(CPSI-MS) in the multiplex monitoring of endogenous metabolites in a trace volume of whole blood was presented in this paper.The fabricated conductive polymer was composed of carbon nanotube(CNT) and polymethyl methacrylate(PMMA).After applying a high voltage of 4.5 kV for generating charged droplet spray,trace volume of extraction solvent(methanol-H2O,1∶1,5 μL) was enough to extract,desorb and ionize over 600 endogenous metabolites and lipids in the only 1 μL of diluted dried blood spot deposited on the tip,covering more than 20 fundamental metabolism pathways including the energy metabolism,amino acids metabolism,nucleotides metabolism,and lipids metabolism,etc.To give a proof of concept illustration,the C57BL/6 mice experiencing the hepatectomy were selected as the model objects.The individual differences in physiological recovery were precisely characterized by capturing hundreds of metabolites changes with a period of 48 h.Finally,a panel of metabolites mainly in the Krebs cycle,arginine metabolism,glycerolipids metabolism,and glycerophosphorylcholine metabolism could give a prognostic reference for the functional recovery of the mice from the hepatectomy. |
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