空气动力辅助离子化质谱组织涂片分析用于胶质瘤异柠檬酸脱氢酶基因状态的快速判别

王子璇; 张雅馨; 回相宜; 张扬; 沈慧聪; 季楠; 贺玖明; 张瑞萍; 厉欣; 马强; 再帕尔·阿不力孜

Rapid Determination of Isocitrate Dehydrogenase Mutations in Human Glioma by Air Flow-assisted Desorption Electrospray Ionization Mass Spectrometry with Tissue Smears Pretreatment

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KeyWord:ambient mass spectrometry tissue smear isocitrate dehydrogenase metabolite analysis air flow assisted desorption electrospray ionization mass spectrometry

Author	Institution
WANG Zi-xuan，ZHANG Ya-xin，HUI Xiang-yi，ZHANG Yang，SHEN Hui-cong，JI Nan，HE Jiu-ming，ZHANG Rui-ping，LI Xin，MA Qiang，ABLIZ Zeper	1.Chinese Academy of Inspection and Quarantine;2.State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College;3.Beijing Tiantan Hospital,Capital Medical University;4.Center for Imaging and Systems Biology,Minzu University of China

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Abstract:

Isocitrate dehydrogenase(IDH) genotype plays a significant role in precise diagnosis of glioma.An air flow assisted desorption electrospray ionization(AFADESI) mass spectrometry(MS) with rapid sample pretreatment using tissue smear instead of tissue cryo section was developed for rapid determination of the gene status of IDH in human glioma.Using intensity ratio of 2 hydroxyglutaric acid to arachidonic acid as an evaluation index,the IDH gene status of glioma tissue could be quickly identified.Quantitative evaluation as well as investigation on the feasibility for ambient mass spectrometry to analyze the metabolites confirmed that the tissue smear method was capable of rapid analysis of metabolites with high simplicity,high repeatability and high coverage.Receiver operating characteristic curve(ROC) demonstrated that the method possessed a strong diagnostic capacity for identifying the gene status of IDH in glioma.Therefore,the developed method could be expected to be a novel technique for precise diagnosis of glioma.