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| Simultaneous Determination of 97 Pesticides in Human Blood by Gas Chromatography-Triple Quadrupole Mass Spectrometry with Simple-QuEChERS Nano |
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| DOI： |
| KeyWord:Simple-QuEChERS Nano gas chromatography-triple quadrupole mass spectrometry human blood pesticides |
| Author | Institution |
| WANG Dan,DONG Lin-pei,REN Xin-xin,JIANG Hong,WANG Ai-hua,CHANG Jing,ZHANG Yun-feng,ZHANG Xi |
1.School of Investigation,Peoples Public Security University of China,Beijing,China;2.Institute of Forensic Science,Ministry of Public Security,Beijing,China;3.Shimadzu Enterprise ManagementChina Co.,Ltd.,Beijing,China |
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| Abstract: |
| A method was established for simultaneous detection of 97 pesticides in human blood by gas chromatography-tandem mass spectrometry(GC-MS/MS) with Simple-QuEChERS Nano.05 mL of blood sample was added to a 15 mL conical bottom polypropylene centrifuge tube and mixed with 15 mL water,then extracted with 20 mL ethyl acetate.The tube was oscillated for 10 min at 20 r/min,and then centrifuged at 8 000 r/min for 10 min.After that,the supernatant of extracts was purified by filtration through a Simple-QuEChERS Nano column and 022 μm organic membrane filter,then detected in multiple reaction monitoring(MRM) mode.There were good linear relationships for all the analytes except fenitrothion,with their correlation coefficients(r2) no less than 0987 3.The limits of detection(LODs) and the limits of quantification(LOQs) for 96 pesticides except allethrin(LOD and LOQ was 1103,3676 ng/mL) ranged from 006 ng/mL to 427 ng/mL and 018 ng/mL to 1424 ng/mL,respectively.At three spiked levels of 100,200,400 ng/mL,the recoveries for 97 kinds of pesticides ranged from 322% to 120%.The intra day precision and inter day precision of the method evaluated at a concentration of 100 ng/mL ranged from 19% to 11% and 36% to 13%,respectively.The developed method,optimized by the traditional QuEChERS method,was not only environmentally friendly,but also improved the efficiency of sample pre treatment and exhibited an enhanced performance in purifying blood sample,which could be used for the qualitative and quantitative analysis of multi pesticides in blood samples of forensic cases. |
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