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| Experimental Techniques and MethodsDetermination of a Novel Anti AIDS Drug ACC007 by High Performance Liquid Chromatography with Ultrasound assisted Ionic Liquid Dispersive Liquid-Liquid Microextraction |
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| DOI： |
| KeyWord:ACC007 ionic liquid dispersive liquid-liquid microextraction high performance liquid chromatography(HPLC) |
| Author | Institution |
| GONG Ai-qin,JIN Dang-qin |
College of Chemical Engineering，Yangzhou Polytechnic Institute,Yangzhou,China |
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| Abstract: |
| In this paper,a simple and efficient method was developed and applied to the separation and determination of ACC007,a novel orally anti AIDS drug in serum samples and its tablets,based on high performance liquid chromatography(HPLC) coupled with ultrasound assisted ionic liquid dispersive liquid-liquid microextraction.The hydrophobic ionic liquid 1 octyl 3 methylimidazolium hexafluorophosphate(［C8mimPF6］) was used as extraction solvent.With the help of ultrasound,the ionic liquid could be divided into fine drops and dispersed entirely into sample solution without any dispersive solvent,promoting the analyte more easily migrating into the ionic liquid phase,and increasing the extraction rate up to 940%.Several important parameters affecting the extraction efficiency were investigated and optimized,including the type and volume of extraction solvent,pH value of sample,volume of sample,ultrasonication time,cooling time and centrifugation time.Under the optimized conditions,the calibration curves were linear over the range of 020-100 μg/mL,with limits of detection(LOD) of 0062 μg/mL for tablet and 0068 μg/mL for serum,respectively.The proposed method was applied to the detection of human serum samples and ACC007 tablets with satisfactory results.The spiked recoveries ranged from 905%to 103%with relative standard deviations of 29%-51%. |
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