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| Simultaneous Determination of Fourteen Polyphenols in Tobacco by Piecewise Detection Based on Liquid Chromatography |
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| DOI： |
| KeyWord:high performance liquid chromatography diode array detector piecewise detection tobacco polyphenols |
| Author | Institution |
| OUYANG Lu-si,LAI Yan-hua,WANG Yu,TAO Hong,HUANG Yi-fei |
Technology Centre,China Tobacco Guangdong Industrial Co.Ltd.,Guangzhou,China |
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| Abstract: |
| A high performance liquid chromatography with diode array detector(HPLC-DAD) was developed for the simultaneous determination of 14 polyphenols,including neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,scopolin,caffeic acid,esculetin,p coumaric acid,ferruric acid,scopoletin,7 hydroxycoumarin,rutin,quercitrin,kaempferol 3 O β rutinoside and quercetin.002 g tobacco powder sample was ultrasonically extracted with 5 mL of 50%methanol for 30 min,and then the supernatant was put to pass through an aqueous phase filtration membrane.14 polyphenols were separated at their optimal detection wavelengths on an Agilent SB-C18 column(30 mm×150 mm，18 μm),with 005 mol/L potassium dihydrogen phosphate aqueous solution and methanol as mobile phases.There existed good linear relationships between peak areas and mass concentrations of the analytes,with their correlation coefficients(r2) larger than 0999 7.The limits of detection and limits of quantitation were in the ranges of 001-015 μg/mL and 003-050 μg/mL,while the intra day and inter day relative standard deviations(RSD) were 070%-36%and 40%-64%,respectively.The spiked recoveries ranged from 952%to 109%.The proposed method could be applied to the simultaneous determination of a variety of polyphenols in tobacco and cigarettes with good sensitivity,precision and recovery. |
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