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| Research on Construction and Application of a Dual-recognition Photoelectrochemical Sensor for Chlorpyrifos Based on BiOI Nanosheets |
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| DOI： |
| KeyWord:BiOI nanosheets chlorpyrifos molecular imprinting acetylcholinesterase（AChE） water sample photoelectrochemical sensor |
| Author | Institution |
| JIN Dang-qin，GONG Ai-qin，WANG Yuan-you，YONG Da-ming，SUN Qiang，ZHU Wen-jun，SUN Yan-yan |
Chemical Engineering Institute， Yangzhou Polytechnic Institute， Yangzhou ， China |
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| Abstract: |
| A chlorpyrifos molecularly imprinted polymer membrane was synthesized by electropolymerization， with BiOI nanosheets as the carrier， chlorpyrifos as the template molecule， p-mercaptoaniline as the functional monomer and glutaraldehyde as the crosslinking agent. Based on this， a dual-recognition photoelectrochemical sensor for chlorpyrifos（CPF）was constructed by immobilizing acetylcholinesterase（AChE） on the surface of the imprinted membrane. The current－time method （I－t） was used to detect chlorpyrifos in water samples.Acetylthiocholine chloride（ATCl） concentration and pH value were optimized. Results showed that，under the optimal experimental conditions（1.0 mmol/L ATCl，pH 7.0）， the peak currents and chlorpyrifos concentrations were linear in the ranges of 0.02－1.0 nmol/L and 20.0－200.0 nmol/L. The linear equations were inhibition rate（%） = 49.253 CCPF + 9.433（r2 = 0.998 6） and inhibition rate（%） = 0.193 CCPF + 57.599（r2 = 0.999 1）， respectively. The detection limit（S/N = 3） was 0.005 nmol/L. The sensor had a good selective recognition ability， reproducibility（RSD = 4.2%） and stability for chlorpyrifos. The method was successfully applied to the detection of trace chlorpyrifos in water samples， with spiked recoveries of 96.1% －108%， and RSDs of 2.8%－4.2%. |
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