|
| Determination of 10 Alkaloids in Refill Solutions for Electronic Cigarettes by Gas Chromatography－Tandem Mass Spectrometry |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:alkaloids liquid－liquid extraction gas chromatography－tandem mass spectrometry refill solutions for electronic cigarettes |
| Author | Institution |
| YAN Jun，WANG Hong-juan，CHEN Zhi-yan，CHEN Huan，ZHOU Yun，LUO Yan-bo，FAN Zhong，FU Ya-ning，DENG Bin-ling，LIU Tong，WEI Ru-dan，HUANG Shi-jie |
1. China Tobacco Guangxi Industrial Co.， Ltd.， Nanning ， China; 2. China National Tobacco Quality Supervision and Test Center， Zhengzhou ， China |
|
| Hits: 1313 |
| Download times: 744 |
| Abstract: |
| A gas chromatography－tandem mass spectrometry（GC－MS/MS） method was established for the simultaneous determination of 10 alkaloids in refill solutions for electronic cigarettes. The instruments and pretreatment conditions were critically examined. The sample was added with 2.0 mL of 2% sodium hydroxide and extracted for 30 min with 10 mL of dichloromethane/methyl alcohol（4∶1， by volume）. The sample extracts were determined by gas chromatography－tandem mass spectrometry under multiple reaction monitoring（MRM） mode after removal of water with anhydrous magnesium sulfate. Results showed that nicotine and nine minor alkaloids presented good linear relationships in the concentration range of 25－1 000 μg/mL and 0.002 5－20 μg/mL， respectively. The linear correlation coefficients were above 0.997， the limits of detection（LOD） ranged from 0.004 0 mg/kg to 0.10 mg/kg， the limits of quantitation were 0.013－0.33 mg/kg， the recoveries ranged from 87.9% to 109% at three spiked levels， and the relative standard deviations（RSDs） for both intra-and inter-day were no more than 6.8% and 7.9%， respectively. The results indicated that the method was sensitive， accurate and reproducible， and suitable for the determination of 10 alkaloids in refill solutions for electronic cigarettes. The method was successfully applied to the analysis of 171 refill solutions for 23 brands. Nicotine levels in refill solutions varied considerably， ranging from 2.16 mg/g to 23.73 mg/g. The median concentrations of nine minor alkaloids were 0.06－7.35 mg/kg， with the detection rate ranging from 64.91% to 99.42%. The proportion of total minor alkaloids to nicotine content in nine electronic cigarette liquid samples was more than 1.0%. |
| Close |