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| Determination of 14 Strobilurin Fungicides Residues in Fruit Wines by Ultra Performance Liquid Chromatography－Tandem Mass Spectrometry with QuEChERS |
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| DOI： |
| KeyWord:ultra performance liquid chromatography－tandem mass spectrometry QuEChERS strobilurin fungicides fruit wines |
| Author | Institution |
| HUANG Xiao-chen，LI Kun，LI Shuo，GUO Ai-jing，WANG Ke，YANG Li-li |
1. College of Chemistry and Material Science，Hebei Normal University，Shijiazhuang ，China; 2. Technology Innovation Center for Chemical Poison Detection and Risk Early Warning， Shijiazhuang Center for Disease Control and Prevention， Shijiazhuang ，China |
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| Abstract: |
| A reliable analytical method was developed for the simultaneous determination of 14 strobilurin fungicides in fruit wines based on ultra performance liquid chromatography－tandem mass spectrometry（UPLC－MS/MS） with QuEChERS. The samples were firstly extracted with acetonitrile， then purified by a modified QuEChERS method（200 mg C18 and 600 mg anhydrous MgSO4）， filtered through a 0.2 μm microporous membrane， and finally determined using the UPLC－MS/MS system. The seperation was performed on a Phenomenex Kinetex F5（3.0 mm × 100 mm， 2.6 μm） column by gradient elution， with acetonitrile and water as the mobile phases. The strobilurin fungicides were determined in positive electrospray ionization mode under multiple reaction monitoring（MRM） mode， and quantified by external standard method. Results showed that good linear relationships for the analytes existed in their respective ranges， with correlation coefficients（r2） all larger than 0.999. The limits of detection（LOD） and the limits of quantitation（LOQ） of the method were in the ranges of 0.002－0.723 μg/kg and 0.006－2.409 μg/kg， respectively. The recoveries for 14 strobilurin fungicides at three spiked levels of 5， 10 and 50 μg/kg ranged from 71.2% to 99.1% with relative standard deviations（RSD， n = 7） of 0.30%－9.4%. This method is simple， efficient， sensitive and accurate， and could be applied to the determination of strobilurin fungicide residues in fruit wine. |
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