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| Determination of Famotidine and Ranitidine in Human Urine by High Performance Liquid Chromatography－Resonance Rayleigh Scattering |
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| DOI： |
| KeyWord:high performance liquid chromatography resonance Rayleigh scattering H2-receptor blockers famotidine ranitidine urine |
| Author | Institution |
| CHEN Fang，ZHANG Yu-yu，LONG Deng-ying，XIAN Hong，PENG Jing-dong |
1. School of Chemical and Materials Engineering， Guiyang College， Guiyang ， China; 2. School of Chemistry and Chemical Engineering， Southwest University， Chongqing ， China |
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| Abstract: |
| A high performance liquid chromatography（HPLC）combined with resonance Rayleigh scattering（RRS） was developed for the determination of two H2-receptor blockers， famotidine（FMTD）and ranitidine（RNTD） in human urine. Separation conditions for HPLC were as follows： a volume ratio of 10 ： 90 for acetonitrile and sodium acetic acid（pH 4.5）， a flow rate of 0.5 mL/min， a Kinetex 5 μm C18 column（250 mm × 4.60 mm）， a sample size of 20 μL and a column temperature of 35 ℃. The optimal experimental conditions were investigated by a new post-column derivation method with metal ion Pd（Ⅱ） and dye erythrin（Ery） as the probes. Results showed that there were good linear relationships for FMTD and RNTD in the ranges of 0.024－25 μg/mL and 0.033－25 μg/mL， with their correlation coefficients（r） of 0.999 0 and 0.998 8， respectively. The detection limits（S/N） for FMTD and RNTD were 7.3 ng/mL and 10.1 ng/mL， respectively. This method was used for the determination of a known concentration of mixed samples. The spiked recoveries ranged from 97.8% to 102%， with relative standard deviations no more than 5.1%. Furthermore， the method was successfully applied to detect the content of FMTD in human urine， without any other matrixes in the urine to interfere the RRS signal. Therefore， the method is reliable and practical， and could be used to detect H2-receptor blockers in human urine. |
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