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| Determination of Tretoquinol in Pungent Spice by Solid-phase Extraction Coupled Ultra-performance Liquid Chromatography－Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:tretoquinol solid-phase extraction ultra-performance liquid chromatography－tandem mass spectrometry（UPLC－MS/MS） pungent spice |
| Author | Institution |
| ZHANG Hai-chao，WANG Jing，JIA Hai-tao，LI Wei，AI Lian-feng，KANG Wei-jun |
1. Shijiazhuang Customs Technology Center，Shijiazhuang ，China; 2. School of Public Health，Hebei Medical University，Shijiazhuang ，China |
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| Abstract: |
| An ultra-performance liquid chromatography－tandem mass spectrometric（UPLC－MS/MS） method was developed for the determination of tretoquinol in pungent spice with MCX solid-phase extraction（SPE） purification. The samples were extracted with 0.2% acid ethanol， purified with a MCX column， and quantified by UPLC－MS/MS in MRM mode after gradient elution on an ACQUITY BEH C18 column with acetonitrile and 0.1% formic acid solution as mobile phase. The results showed that the calibration curves were linear in the range of 0.1－50 ng/mL with correlation coefficients（r） more than 0.998. The limits of detection （LODs） were 0.2 μg/kg and the limits of quantitation （LOQs） were 0.5 μg/kg. The average recoveries of tretoquinol at four different spiked levels （0.5， 2， 10， 100 μg/kg） ranged from 74.5% to 98.8%，with relative standard deviations（RSD） of 3.1%－9.1%. The proposed method is sensitive， accurate and reliable， and could be used for the determination of tretoquinol in pungent spice. |
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