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| Determination of Carvedilo in Biological Samples Using a Fluorescence Spectropho? tometry Coupled with Magnetic Solid Phase Extraction |
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| DOI： |
| KeyWord:carvedilol magnetic solid phase extraction covalent-organic frameworks fluorescence spectrophotometry plasma urine |
| Author | Institution |
| QIN Yan-fang，ZHAO Bing-xin，LI Xiao-jiao，XU Feng-yun，WU Hao |
1. Changzhi Preschool Teachers College，Changzhi ，China;2. School of Chemistry and Materials Science，Shanxi Normal University，Linfen ，China |
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| Abstract: |
| In this work，Fe3O4@COF（TpBD）magnetic composites were successfully synthesized through the reaction of Fe3O4 nanoparticles，1，3，5-trimethylphloroglucinol（Tp）and benzidine（BD） via a solvothermal approach. The fabricated magnetic nanocomposites were applied as the adsorbent for magnetic solid phase extraction（MSPE）of carvedilo（CAR）. Coupled with fluorescence spectro? photometry，a new analytical method of MSPE was developed for the determination of CAR in biologi? cal samples. The Fe3O4@COF（TpBD）absorbent was characterized by transmission electron microsco? py（TEM），X-ray diffraction（XRD），Fourier transform infrared spectrometry（FT－IR），vibrating sample magnetometry（VSM）and surface area and pore size analyzer. The experimental parameters were optimized，including pH value of solution，amount of adsorbent，adsorption time，the type and volume of desorption solvent and desorption time. The optimum MSPE conditions were as follows：a solution pH value of 6. 0，an adsorbent amount of 5 mg，an extraction time of 4 min，and a desorption time of 5 min. Under the optimum conditions，the method exhibited good linearity in the concentra? tion range of 1－100 ng/mL. Moreover，the limits of detection（S/N = 3）and quantification（S/N = 10）were 0. 19 and 0. 63 ng/mL，respectively. Recoveries at low，medium and high spiked levels were in the range of 95. 9%－98. 7%. With the advantages of good recovery，high sensitivity and good stability，the proposed method could be applied to the analysis of CAR in plasma and urine samples. |
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