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| Detection of Mercury by a Label-free Aptamer-based Fluorescent Assay |
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| DOI： |
| KeyWord:aptamer mercury rapid detection fluorescent assay PicoGreen |
| Author | Institution |
| WANG Nan，CHEN Jia-yi，LU An-xiang，LUAN Yunxia，LU Jing-hua |
1. College of Pharmacy，Jinzhou Medical University，Jinzhou ，China;2. Beijing Research Center for Agricultural Standards and Testing，Agricultural Product Quality and Safety Risk Assessment Laboratory of the Department of Agriculture，Beijing Municipal Key Laboratory of Agriculture Environment Monitoring，Beijing ，China |
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| Abstract: |
| In this paper，a rapid fluorescence assay for the detection of mercury ions was developed using mercury－specific aptamer and PicoGreen，a fluorescent dye embedded in double helix DNA. Mercury ion concentration was determined by fluorescence signal intensity，and the linear relation? ship between the logarithm of mercury ion concentration and fluorescence intensity was obtained. The linear range of the method was 0. 001-100 μg/mL，and the detection limit was 0. 38 ng/mL. In the presence of interference ions such as As3+ ，Pb2+ ，Cd2+ ，Cr2+ ，K+ ，it has a good specificity. Mean? while，the irrigation water samples with different concentrations of Hg2+ showed a good recovery of 95. 2%－97. 9% and the relative standard deviation of 2. 3%－4. 0%. The method is simple，rapid and sensitive，and is applicable for the determination of mercury ions by using aptamer and fluores? cent dye. |
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