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| Application Advances of Paper-based Mi? crofluidic Analytical Devices in Food Safety Inspection |
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| DOI： |
| KeyWord:microfluidic paper-based analytical devices(μPADs) ?food safety ?rapid detection ?review |
| Author | Institution |
| LI Lu，YANG Jin-yi，XU Zhen-lin，WANG Hong， LEI Hong-tao，SUN Yuan-ming，SHEN Yu-dong |
Key Laboratory of Food Quanlity and Safety of Guangdong Province，College of Food Science，South China Agriculture University，Guangzhou ，China |
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| Abstract: |
| Paper-based microfluidic analytical devices（μPADs）are a kind of technology for sample preparation， reaction， separation， and detection on paper chips， which have been widely used in biomedical， environmental， clinical analysis， chemical and other fields owing to their advantages， such as inexpensive production， simple fabrication， easy recycle， diverse structures， low reagent consumption， environmentally-friendly and biodegradation. In addition， they have the advantage of high sensitivity， which could be used by non-professionals to obtain the test results quickly，so they also exhibit a great practical value and a development potential in the rapid detection of food safety. This review highlights the recent advances on fabrication of paper chips， fluid transportation and control， and signal detection modes from the technical level. Firstly， this paper roughly describes the functional modification methods for paper chips with rich hydroxyl groups and high hydrophilicity， such as lithography， wax techniques， inkjet printing， screen printing， flexographic printing， 3D printing， stamping， polydimethylsiloxane（PDMS）and other methods. Then three immobilization ways of biosensor molecules on paper-based materials， including physical adsorption， embedding and chemical immobilization are described. According to the direction of fluid flow（horizontal direction or vertical horizontal direction）， the methods for preparing the processed paper into two-dimensional（2D）or three-dimensional（3D）paper chips are summarized. Secondly， the transport mechanism of fluid in two stages of incomplete soaking and complete soaking on paper based materials is discussed，incomplete soaking stage refers to the fluid on the paper based materials in the slow penetration of the paper， while complete soaking stage is when the fluid fills the paper based materials and is transported by capillarity. Moreover， this paper comprehensively analyzes the intelligent fluid manipulation technology， which can transport reagents and samples to the corresponding area within the appropriate detection time. Fluid transportation and control methods can be divided into passive device and active device. Passive device is simple and easy to manufacture without additional equipment， while active device relies on external input system and can achieve accurate multi-step fluid flow control. Finally， this review introduces several detection methods that could be combined with paper based microfluidic platform in detail， such as colorimetric， electrochemical， chemiluminescence and electrochemiluminescence， fluorescence， etc. Furthermore， the research progress of paper-based microfluidic technology in food hazardous molecules， foodborne pathogens， heavy metal contamination and other aspects is reviewed， and the challenges and development trends of paper-based microfluidic technology in the food industry are also proposed. |
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