|
| Study on a Porous Organic Cage Chiral Column for Capillary Electrochromatography |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:porous organic cages chiral stationary phase capillary electrochromatographic column chiral separation |
| Author | Institution |
| YU Zi-yun，TANG Ming-hua，ZHANG Jun-hui，YUAN Li-ming |
Faculty of Chemisty and Chemical Engineering，Yunnan Normal University，Kunming ，China |
|
| Hits: 810 |
| Download times: 586 |
| Abstract: |
| A porous organic cage（POC） with large specific surface area，uniform porosity and tetrahedral structure was synthesized with（1R，2R）-diphenylethylenediamine and 2-hydroxy-1，3，5-pyromellitic formaldehyde．The porous material was characterized by X-ray powder diffraction，nuclear magnetic resonance spectrometry，Fourier transform infrared spectroscopy，thermogravimetric analysis，scanning electronic microscopy and nitrogen adsorption determinations．Meanwhile，a capillary electrochromatographic column was prepared by using the POC as chiral stationary phase．After optimizing the operating voltage，buffer solution concentration and pH value，the chiral recognition performance of the column was investigated in capillary electrochromatography．The experimental results showed that the chiral column could separate dihydroflavonoids and ketoprofen chiral compounds，as well as o，m，p-nitrophenol and o，m，p-nitroaniline positional isomers．The optimized conditions for separation of dihydroflavonoids and ketoprofen were：100 mmol/L buffer solution，pH values of 4.5 and 3.5，separation voltages of 16 kV and 14 kV，respectively．The relative standard deviations（RSDs） of the retention time were 1.2% for five consecutive injections of ketoprofen on the column and 4.0% for three consecutive days．The POC chiral capillary electrochromatographic column possesses a good stability and reproducibility．The POC is a excellent chiral stationary phase，which indicates a great prospect in the resolution of chiral drugs and positional isomers． |
| Close |