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| Detection of Vibrio Parahaemolyticus by an Aptameric Fluorescence Method Based on Metal Organic Framework Material and Enzyme-assisted Signal Amplification |
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| DOI： |
| KeyWord:Vibrio Parahaemolyticus（V.P） aptamer metal organic frameworks exonuclease fluorescence method |
| Author | Institution |
| FENG Run-chan，YUAN Ping TAN，Shu-liang，WU Fu-hai，SU Zheng-quan，BAI Yan，HE Jin-can |
School of Public Health，Guangdong Pharmaceutical University，Guangzhou ，China |
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| Abstract: |
| Based on the fluorescence quenching property of copper-based metal organic framework（Cu－MOF），the selective recognition ability of aptamers and the catalytic hydrolysis of exonuclease，a rapid aptameric fluorescence method was developed for the detection of Vibrio parahaemolyticus（V.P）．The Cu－MOF was prepared at room temperature．The morphology，structure and fluorescence quenching mechanism of Cu－MOF were systematically studied．The analytical conditions such as volume of Cu－MOF，quenching time，volume of exonuclease，recovery time and addition order of agents were investigated．Based on the optimized conditions，an aptameric fluorescence method was established for the detection of V.P．There was a linear relationship between logarithm of concentration（lgc） and increase of fluorescence recovery intensity（?F） in the range of 8.44 × 102－2.03 × 105 CFU/mL，with a correlation coefficient（r2） of 0.998 6 and a linear equation of ?F = 7.30lgc－19.58．The detection of limit was 37 CFU/mL．The method could be carried out within 30 min．The proposed method was applied to the determination of V.P in shrimp and octopus samples with recoveries of 88.8%－109%．The method is rapid，sensitive and selective，and has a certain application potential in the analysis of V.P in complex samples. |
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