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| Determination of γ-Hydroxybutyric Acid in Blood by MPFC－QuEChERS with GC－MS/MS |
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| DOI： |
| KeyWord:γ-hydroxybutyric acid MPFC－QuEChERS gas chromatography-tandem mass spectrometry blood |
| Author | Institution |
| LI Run-kang，LI Jia-yi，ZHANG Yun-feng，DONG Lin-pei，HE Hong-yuan，WEI Chun-ming |
1. Institute of Forensic Science，Ministry of Public Security，Beijing ，China; 2. School of Investigation，People’s Public Security University of China，Beijing ，China |
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| Abstract: |
| A new method was developed for the determination of γ-hydroxybutyric acid（GHB） in human blood by gas chromatography－tandem mass spectrometry（GC－MS/MS） with a kind of purification column（MPFC－QuEChERS）．Effects of sample pretreatment conditions and analytical instrument conditions on the test results were investigated in the round，and the optimal experimental conditions were determined. First of all，GHB－D6 was spiked into 0.1 mL blood sample as the internal standard，and then the analyte was extracted using ethyl acetate efficiently．After that，the supernatant was allowed to flow through the MPFC－QuEChERS purification column and evaporated to dryness．Finally，the dried residue was derivated with trifluoroacetamide（BSTFA） and analyzed in multi-reaction monitoring（MRM） mode．Experimental results showed that there were linear relationships for the spiked human blood samples in the range of 0－1 000 ng/mL（r2 > 0.995 1）．The limits of detection（LOD） and limits of quantitation（LOQ） of the method were 0.2 ng/mL and 0.5 ng/mL，respectively．The inter-day relative standard deviations（RSD） and intra-day RSD were both less than 15%．An extraction recovery of 80.1%－84.7% was obtained for the spiked human blood samples．The matrix effect ranged from 87.4% to 94.4%．The results indicated that the developed method was improved and optimized by traditional liquid－liquid extraction method，it could be applied to the rapid and effective determination of GHB in human blood samples of actual cases with its good stability and high sensitivity. |
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