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| Advances on?in Vitro?Detection and?in Vivo?Imaging of Alkaline Phosphatase |
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| DOI： |
| KeyWord:alkaline phosphatase ?colorimetry ?electrochemistry ?surface enhanced Raman scattering ?fluorescence analysis ?bioimaging |
| Author | Institution |
| YUAN Zhen-zhen，SUN Ya-wei，ZHANG Qian，ZHANG Chun-yang |
College of Chemistry，Chemical Engineering and Materials Science，Shandong Normal University， Jinan ，China |
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| Abstract: |
| Alkaline phosphatase（ALP） is a hydrolase that can catalyze the removal of the phosphate group from proteins，nucleic acids and carbohydrates．ALP widely exists in both prokaryotes and eukaryotes and plays an important role in regulating various biological processes such as cell division，proliferation，apoptosis and signal transduction．The dysregulation of ALP activity is closely associated with a variety of human diseases including cardiovascular diseases，diabetes and cancers，making it a promising biomarker for disease diagnosis．Thus，sensitive detection of ALP activity is essential to clinical diagnosis and biomedical research．In this review，the emerging strategies for?in vitro detection and in vivo imaging of ALP in recent years are summarized．The in vitro detection methods include colorimetry，electrochemistry，surface enhanced Raman scattering and fluorescence measurements，while the in vivo imaging methods mainly rely on fluorescence imaging．Moreover，new insights into the challenges and future directions of ALP assay are given． |
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