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| Electrochemical Immunosensor Based on Au－Ag Bimetallic Nanoclusters@CNTs－TiO2 Nanomaterials as Novel Redox Probes |
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| DOI： |
| KeyWord:bimetallic nanoclusters ?synergistic effect ?Au－Ag NCs@CNTs－TiO2?nanocomposites ?carbohydrate antigen 19－9 |
| Author | Institution |
| HUANG Xiao-mei，DENG Xiang，DENG Zi-he |
1. Department of Chemistry and Chemical Engineering，Sichuan University of Arts and Science，Dazhou ，China;
2. Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province，Dazhou ，China;
3. Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education，Zigong ，China;
4. College of Chemistry and Chemical Engineering，Shihezi University，Shihezi ，China |
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| Abstract: |
| A highly sensitive "sandwich" electrochemical immunosensor was constructed for the detection of carbohydrate antigen 19－9（CA19－9），by synthesizing a unique "pearl necklace" structure of gold-silver bimetallic nanoclusters multi-walled carbon nanotubes-titanium dioxide nanocomposites（Au－Ag NCs@CNTs－TiO2 NPs） used as a signal label，carrier and signal amplifier．Firstly，the magnetic Au－Fe3O4?NPs with peroxidase mimetic properties were used to modify the electrode，then the anti－CA19－9 antibody and BSA were immobilized on the electrode surface through chemical bonding，and subsequently，the antibody was bound by specific reaction．Finally，Au－Ag NCs@CNTs－TiO2 NPs/Ab2/BSA biological coupling with redox activity and amplification was combined to form a sandwich immune structure．The experimental results showed that the response signal was effectively amplified by using double peroxide analog enzyme to catalyze H2O2 in the substrate. Under the optimal experimental conditions，CA19－9 showed a good linearity in the concentration range of 0.01－200 U/mL，with a detection limit of 0.003 U/mL．The recoveries of the immunosensor ranged from 94.6% to 105%，with relative standard deviations（RSD） of 4.3%－6.5%，indicating that the immunosensor could be used for the detection of CA19－9 in clinical serum samples. |
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