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| Preparation and Identification of Reference Materials of Isorhamnetin and Rutin in Seabuckthorn Fruit Powder |
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| DOI： |
| KeyWord:reference materials ?seabuckthorn ?isorhamnetin ?rutin ?preparation and identification |
| Author | Institution |
| YANG Xiao，YANG Jing-ying，PAN Ming-fei，SONG Yang，WANG Shuo |
1. State Key Laboratory of Food Nutrition and Safety，Tianjin University of Science & Technology，Tianjin ，China;2. Tianjin Key Laboratory of Food Quality and Health，Tianjin University of Science and Technology，Tianjin ，China;3. College of Life Sciences，Tianjin Normal University， Tianjin ，China |
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| Abstract: |
| Isorhamnetin（IS） and rutin（RU），two flavonoid compounds present in seabuckthorn，have very strong antioxidant activities for effective removal of free radicals in human body．In this paper，IS and RU of high-purity were prepared by a high-pressure liquid phase preparation method with ethanol dissolution，then their chemical structures were identified by Fourier transform infrared spectroscopy（FT－IR），ultraviolet（UV） spectroscopy，mass spectrometry（MS） and H/C－nuclear magnetic resonance（H/C－NMR）．Furthermore，a typical HPLC method was used to evaluate the obtained IS and RU products in terms of homogeneity（inter and intra-bottles） and stability（long-term：6 months，－20 ℃；short-term：1 week，4 ℃ and －20 ℃），both of them were confirmed excellent by statistical data．Finally，the purities of the prepared IS and RU reference materials were jointly determined by multiple laboratories（n = 8） to be 99.16% and 99.22%，with their extended uncertainties of 0.14% and 0.13%，respectively．The IS and RU reference materials obtained in the study could be used as reliable references in drug analysis or quality evaluation on seabuckthorn products．This study provided an effective method for the preparation and identification of IS and RU reference materials in seabuckthorn，which could be applied to the preparation of other reference materials． |
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