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| Detection of Lornoxicam by Using a Novel Hyacinth Beans Carbon Quantum Dots/Fluorescein Isothiocyanate Based Radiometric Probe |
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| DOI： |
| KeyWord:hyacinth beans carbon quantum dots（HB－CQDs） ?fluorescein isothiocyanate（FITC） ?fluorescence ratiometric probe ?lornoxicam（LNXC） |
| Author | Institution |
| SUN Ling-bo，WU Wen-bo，WANG Qing-qing，ZHANG Yue-cheng，MA Hong-yan |
1. Medical College，Yan'an University，Yan'an ，China;2. College of Chemistry and Chemical Engineering，Yan'an Key Laboratory of Analytical Technology and Detection， Yan'an University，Yan'an ，China |
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| Abstract: |
| A novel hyacinth beans carbon quantum dots（HB－CQDs） with nature hyacinth beans as the single carbon source were synthesized by hydrothermal method，which exhibit excellent fluorescent properties and water solubility．Fluorescein isothiocyanate（FITC） was composited with HB－CQDs in HAc－NaAc buffer（pH = 5.80） to form HB－CQDs/FITC composites，which could clearly show two fluorescence emission peaks at 400 nm and 510 nm under the single excitation wavelength of 326 nm，whereas both fluorescence signals were“switch off”after MnO?44- was added into the system．With the addition of lornoxicam（LNXC），the fluorescence signal at 510 nm was restored，which makes the fluorescence signal“switch on”again，even though the fluorescence intensity at 400 nm was unvaried．Thus，a novel approach for the detection of LNXC based on HB－CQDs/FITC fluorescent ratiometric probe was established．The quenching mechanism of MnO?44- was further explored．A good linear relationship between the concentration of LNXC and the fluorescence intensity ratio（at λem/λem = 510 nm/400 nm） of HB－CQDs/FITC probe was found under the optimized experimental conditions，with a linear range of 1.0 × 10－7－1.0 × 10－5 mol/L．The detection limit of this method was 9.0 × 10－8 mol/L. Furthermore，this probe was used in the detection of the content of LNXC in the samples with satisfactory results． |
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