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| Highly Sensitive Detection of Luteolin Using a Gold Nanoparticles Modified Carbon Fiber Microelectrode |
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| DOI： |
| KeyWord:luteolin ?gold nanoparticles（AuNPs） ?electrochemical sensor |
| Author | Institution |
| SHI Min，BAO Chang-hao，MA Wen-wen，CHENG Han |
1. School of Pharmaceutical Sciences，South-Central University for Nationalities，Wuhan ，China;2. National Demonstration Center for Experimental Ethnopharmacology Education，South-Central University for Nationalities，Wuhan ，China |
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| Abstract: |
| An electrochemical method for the determination of luteolin（Lu） was established in this paper．Gold nanoparticles（AuNPs） were prepared by reducing chloroauric acid with trisodium citrate，which was then electrodeposited on the surface of carbon fiber microelectrode（CFME） by one-step potentiostatic deposition. A simple and sensitive electrochemical sensor for the determination of luteolin was constructed．Cyclic voltammetry（CV），electrochemical impedance spectroscopy（EIS） and differential pulse voltammetry（DPV） were used to investigate the catalytic performance of the modified electrode for the electrochemical oxidation of luteolin. The results showed that the AuNPs/CFME had an obvious electrocatalytic performance for the detection of luteolin， and the optimal modification time of AuNPs was 30 min. There were good linear relationships between peak currents and luteolin concentrations in the ranges of 0.01－0.10 μmol/L and 0.10－10 μmol/L，with their correlation coefficients（r2） of 0.994 4 and 0.995 1，respectively．The limits of detection（LODs，S/N?= 3） were 1.58 nmol/L. With the advantages of simple fabrication，good stability，high sensitivity and excellent anti-interference ability，the developed sensor could be applied to the quantitative determination of luteolin in Kudo capsules．The spiked recoveries for the analyte ranged from 96.0% to 104%，with relative standard deviations（RSDs） less than 5.0%．It had a good application prospect in quantitative analysis of luteolin in biological microenvironment. |
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