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| Determination of Nine Cannabinoids in Incense Smoke by Gas Chromatography－Mass Spectrometry Combined with Solid Phase Extraction－Isotope Dilution |
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| DOI： |
| KeyWord:burnable incense ?smoke ?cannabinoids ?gas chromatography－mass spectrometry（GC－MS） |
| Author | Institution |
| TANG Qing-qiang，WU Chun-deng，CAO Xiao-gang，WANG Jun，CHEN Di，YANG Fang |
1. Technology Center of Fuzhou Customs District，Fuzhou ，China;2. Technology Center of Lhasa Customs District，Lhasa ，China |
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| Abstract: |
| A comprehensive analytical method based on gas chromatography－mass spectrometry（GC－MS） was developed for the determination of 9 cannabinoid compounds in burnable incense smoke．The smoke of burnable incense sample was extracted with a sampling pump，then enriched into the solvent in a tube．Finally，the solvent was concentrated and centrifuged at 10 000 r/min for 5 min．The extract was purified on an HLB solid phase extraction column with water containing 10% methyl alcohol as the eluent．The sample was eluted with 6 mL methyl alcohol，and then filtered through a 0.22 μm membrane filter unit prior to GC－MS analysis．Temperature－programmed was applied at an initial temperature of 70 ℃．After 2 min，the temperature was raised to 180 ℃ at a rate of 10 ℃/min．After 18 min，the temperature was raised to 300 ℃ at a rate of 15 ℃/min．9 cannabinoid compounds were separated on an Agilent HP－5MS column（30 m × 0.25 mm × 0.25 μm）．Identification and quantification were achieved using an electrospray ionization（EI） source under the selected ion monitoring（SIM） mode．The internal standard method was used to quantify the cannabinoids．The results indicated that the 9 compounds could be analyzed within 27 min．The calibration curves showed good linearities for 9 cannabinoid compounds in the range of 0.01－4.0 mg/L， with correlation coefficients（r2） not less than 0.999 5．The limits of detection（LODs，S/N＝3） and limits of quantitation（LOQs，S/N＝10） for 9 cannabinoid compounds were in the range of 0.003－0.06 mg/L and 0.01－0.2 mg/L，respectively．The average recoveries for 9 cannabinoid compounds were in the range of 74.8%－114%，with relative standard deviations（RSDs，n＝6）of 1.6%－9.1%．This method was used to determine 10 types of burnable incense，no new psychoactive substances of cannablis were detected．The method is simple to operate，and could be used for rapid qualitative and quantitative analysis of cannabinoid compounds in burnable incense smoke. |
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