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| Comparison of Chemical Constituents in?Dioscorea polystachya Truczaninow cv. Tiegun Planted in Different Counties by 1H NMR Metabonomics Approach |
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| DOI： |
| KeyWord:Dioscorea polystachya Truczaninow ?authentic medicinal materials ?nuclear magnetic resonance （NMR） ?multivariate statistical analysis ?glucose ?fructose |
| Author | Institution |
| WEI Liang，ZHANG Chao-nan，LI Ke-yan，WEI Ting-ting，QI Shao-ke，WANG Yan-li，WANG Qiang |
1. National Health Commission Key Laboratory of Birth Defect Prevention，Henan Institute of Reproductive Health Science and Technology，Zhengzhou ，China;2. High & New Technology Research Center of Henan Academy of Science，Zhengzhou ，China;3. School of Pharmacy and Chemical Engineering， Zhengzhou University of Industrial Technology，Zhengzhou ，China;4. Henan GRG Metrology & Test Co.，Ltd.，Zhengzhou ，China |
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| Abstract: |
| Geo-authentic Chinese medicinal materials refer to those traditional Chinese medicines that have been verified through long-term clinical application and produced in a specific region．Huai Shanyao is a class of geo-authentic Chinese medicinal materials，while?Dioscorea polystachya Turczaninow cv.?Tiegun is considered the best Huai Shanyao as its price is the highest. So the dealers often adulterate?D.polystachya cv.?Tiegun planted in other places or other yams with similar appearance as the authentic?D.polystachya cv.?Tiegun planted at Wenxian county，Henan Province to earn higher profits．In the previous study，it was found that?D.polystachya cv.?Tiegun planted at Wenxian county could be verified according to the contents of glucose and fructose，and the tetrazolium solution could be used as an identification reagent for the rapid discrimination of?D.polystachya cv.?Tiegun from Wenxian county based on the color reaction of glucose．In fact，not only?D.polystachya cv．Tiegun from Wenxian county，but also the yams from other three counties（Mengzhou，Qinyang and Wuzhi） in Jiaozuo city，Henan Province belonged to Huai Shanyao．For the identification of Huai Shanyao，seven groups of D.polystachya Truczaninow cv.?Tiegun were analyzed by NMR-based metabolomics appraoach，and 30 metabolites were identified．The metabolites of different yams were analyzed using principal component analysis（PCA） and orthogonal projections to latent structures－discriminant analysis（OPLS－DA）．Multivariate analysis revealed that yam planted in loessial soils at Wenxian county（WL） had the highest level for arginine and the lowest level for sucrose．Meanwhile，Mengzhou loessial soil yam（ML） had the highest level of threonine and glutamine，Wenxian sandy soil yam （WS） had the highest level of alanine and malate，and Wuzhi sandy soil yam（ZS） had the highest level of allantoin．The level of 2-hydroxyisobutyrate in sandy soil yams is higher than loessial soil yam．Loading plots of OPLS－DA showed the variable important projections（VIPs） for glucose and fructose were both lower than 1.0，indicating that glucose and fructose were not the principle components．Contents of citrate，malate and sucrose were determined by high performance liquid chromatography（HPLC），and the results obtained were consistent with the loading plots of OPLS－DA．Glucose and fructose were undetectable by HPLC（LOD：2.0 mg/g） in all yams．All these results showed that geo-authentic Chinese yams（Huai Shanyao） could be verified according to the contents of glucose and fructose．And the glucose chromogenic reagents could be used as an indicator for the rapid discrimination of geo-authentic Chinese yams．This study reveals the metabolites difference in Huai Shanyao planted in different counties，as well as that the characteristic metabolites are the rationale for the authentication of geo-authentic Chinese yams． |
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