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| Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection |
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| DOI： |
| KeyWord:honey sugar high performance liquid chromatography evaporative light scattering detection |
| Author | Institution |
| JIA Ru，HUANG Xue-zhe，JIA Guang-qun，SHI Yu-qiu，CUI Zong-yan，GUO Ze-bin |
1. College of Food Science，Fujian Agriculture and Forestry University，Fuzhou ，China; 2. Technology Center of Qinhuangdao Customs，Qinhuangdao ，China |
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| Abstract: |
| An ultra performance liquid chromatography（UPLC） coupled with evaporative light scattering detection（ELSD） was established for the determination of 12 saccharides，i.e．fructose，glucose，sucrose，turanose，mlatulose，maltose，kojibiose，isomaltose，erlose，melezitose，raffinose and maltotriose in honey. Honey sample was dissolved in deionized water and kept constant volume with acetonitrile．The solution was then filtered with a 0.22 μm filter membrane．12 saccharides were separated on an Amide column，then determined with an evaporative light scattering detector，and finally quantified by external standard calibration method．Through the optimization experiment，there existed good linear relationships for 12 saccharides，and the linear correlation coefficients were in the range of 0.997 7－0.999 8．The obtained results for fructose and glucose by this method were comparable to those of the traditional refractive index detection method．The average recoveries for the other 10 saccharides ranged from 90.0% to 105%，and the relative standard deviations were between 0.70% and 5.8%．The method was validated to be sensitive，accurate and repeatable．It was suitable for simultaneous quantitative analysis of multiple sugar components in honey．The proposed method was applied to the determination of 12 saccharides in 72 batches of honey samples of different botanical origins．It was found that turanose，mlatulose and maltose are the dominant disaccharides in Chinese honeys．This method and test data provided an important support for honey quality and quality evaluation． |
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