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| Rapid Analysis of Chemical Components in Zuogui Jiangtang Shuxin Formula by UHPLC - Q - Exactive - Orbitrap Mass Spectrometry |
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| DOI： |
| KeyWord:Zuogui Jiangtang Shuxin formula ultra performance liquid chromatography－quadrupole－exactive orbitrap mass spectrometry chemical components small peptides |
| Author | Institution |
| HUANG Ya-lan，ZHANG Yan-ling，XIANG Qin，YU Rong，WU Yong-jun，LIU Xiu |
1. The First Hospital of Hunan University of Traditional Chinese Medicine，Changsha ， China; 2. College of Traditional Chinese Medicine， Hunan University of Chinese Medicine， Changsha ， China; 3. Science and Technology Department of Hunan University of Traditional Chinese Medicine，Changsha ， China; 4. Graduate School of Hunan University of Traditional Chinese Medicine， Changsha ， China; 5. School of Pharmacy， Hunan University of Chinese Medicine， Changsha ， China |
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| Abstract: |
| An ultra performance liquid chromatography－quadrupole－exactive orbitrap mass spectrometry（UHPLC－Q－Exactive－Orbitrap－MS）was developed for the rapid analysis of the chemical components in Zuogui Jiangtang Shuxin formula．The aqueous decoction of Zuogui Jiangtang Shuxin formula was separated on an Xbridge BEH C18（2.1 mm × 100 mm，2.6 μm） column with 0.1% formic acid－water and acetonitrile as mobile phase．The eluent was detected by quadrupole orbitrap high resolution mass spectrometry in ESI positive and negative ion modes．The raw data was processed using Xcalibur 4.3 and Compound Discoverer 3.2 software．A total of 290 chemical components were tentatively identified from the aqueous decoction of Zuogui Jiangtang Shuxin formula，including 224 known compounds with structure classes of flavonoids，phenylpropanoids，phenols，saponins，organic acids，alkaloids，nucleosides，iridoids and oligoses．It is worth noting that the other 66 compounds were detected and identified from Zuogui Jiangtang Shuxin formula for the first time，which were mainly amino acids/small peptides．This developed method could be used for rapid and accurate analysis of the chemical components in Zuogui Jiangtang Shuxin formula，which could provide a reference for further research on the therapeutic material basis of Zuogui Jiangtang Shuxin formula and guiding the rational use of drugs in clinical practice as well as later experimental studies． |
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