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| Serum Lipidomic Analysis of Colorectal Cancer Patients by Ultrahigh Performance Liquid Chromatography Tandem Q Exactive Orbitrap Mass Spectrometry |
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| DOI： |
| KeyWord:colorectal cancer ultra-high performance liquid chromatography tandem Q exactive orbitrap mass spectrometry lipidomics serum biomarkers |
| Author | Institution |
| CHEN Hong-wei，NONG Yan-ying，ZHU Yi-fan，LIANG Yun-xiao，ZHOU Hai-lin，ZHANG Jia-hao，ZHANG Qi-song |
1. Medical College of Guangxi University，Nanning ，China; 2. Department of Gastroenterology，Ruikang Hospital Affilated to Guangxi University of Chinese Medicine，Nanning ，China; 3. Department of Gastroenterology，People’s Hospital of Guangxi Zhuang Autonomous Region， Nanning ，China |
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| Abstract: |
| Colorectal cancer（CRC） is one of the most malignant tumors in the world and the main cause of cancer death．Traditional diagnostic methods for CRC patients have poor performance owing to their low sensitivity or specificity．The aims of this study are to explore the lipid biomarkers and the metabolic pathways of CRC for better understanding of its pathogenesis as effective and convenient biomarkers for CRC diagnosis are still lacking，and there are relatively few of serum lipidomic study on CRC for the time being．Therefore，the lipidomics analysis on serum samples from 50 normal controls and 50 CRC patients was performed by ultra-high performance liquid chromatography tandem Q Exactive orbitrap mass spectrometry．Lipidomic data was analyzed by univariate statistical analysis，including Mann－Whitney U or Student’t test and multiple statistical analysis，including principal component analysis（PCA） and orthogonal partial least squares discriminant analysis（OPLS－DA）．In addition，the established OPLS－DA model was validated by 200 times permutation test to exam its fitness and reliability．Through the comparison of lipid profiles，there were significant differences in the serum lipidomics profiles between two groups，and 155 differential lipids were found responsible for their discrimination based on P < 0.05 and fold change > 1.5 or < 0.67．Most of them belonged to phosphatidylcholine（PC） and triacylglycerol（TAG） with constituent ratio of 30.32% and 27.10%，respectively，indicating that dysregulation of PC and TAG metabolism could be closely related with CRC formation．Then，the diagnostic ability of differential lipids was evaluated by receiver operating characteristic curve（ROC） analysis．A total of 9 differential lipids with high specificity and sensitivity as well as high diagnostic performance（AUC > 0.80） for CRC diagnosis were selected and identified by using mzCloud and LipidBlast database，including the species of palmitoyl ethanolamide，palmitic acid，sphingosine，sphingomyelin（SM）d40∶3，SM d36∶0，TAG 58∶1，PC 34∶2，PC 36∶6，PC 38∶7．These lipid species，served as the potential diagnostic biomarkers of CRC，exibited a great clinical application potential in serum-based lipidomics screening of CRC．Meanwhile，the study also provided some basic data that could be used to profile the lipid characteristics and explore the mechanism of CRC. |
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