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| Detection of Moisture Content in Tobacco Based on Rhein Carbon Dot Fluorescent Probe |
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| DOI： |
| KeyWord:carbon dots moisture determination rhein smartphone colorimetry fluorescent probe |
| Author | Institution |
| SHEN Shao-yi，LI Bin，YANG De-zhi，YANG Ya-ling |
1. Tobacco Leaf Production Center，Yunnan Tobacco Leaf CO.，LTD，Kunming ，China; 2. Faculty of Life Science and Technology，Kunming University of Science and Technology，Kunming ，China |
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| Abstract: |
| Mg2+ and rhein were used as precursor materials to prepare rhein-carbon dots. Based on the quenching effect of moisture on the fluorescence signal of rhein-carbon dots，a simple and rapid method for the determination of moisture content in tobacco leaf processing was established with the help of smartphone RGB signal acquisition technique. The fluorescence quenching mechanism showed that the interaction between the protic solvent（water） and the aprotic solvent（DMSO） could significantly quench the fluorescence signal of rhein-carbon dots. There was a good linear relationship between fluorescence quenching effect and the water content in the range of 30－1 000 mg，with a detection limit of 5 mg. Using a smartphone to collect signals and reading RGB values through software，the results showed that there was a good linear relationship between the water content and the R value within the linear range （r2 = 0.990 2）. The method could realize rapid on-site determination of water content in tobacco leaf processing，and the results met the actual determination requirements. With the characteristics of simplicity，stability and accuracy，this method provides a new research strategy for water detection techniques and related probe design. |
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