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| Construction of a Carbon Dot/MnO2 Nanoprobe for Detection of Anti-tuberculosis Drug Isoniazid |
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| DOI： |
| KeyWord:CDs/MnO2 nanoprobe isoniazid（INH） fluorescence resonance energy transfer（FRET） anti-tuberculosis drug |
| Author | Institution |
| WANG Xin-xu，FAN Bing-jun，YAN Xiao-hui，WANG Ning |
1. First Clinical Medical College，Shanxi Medical University，Taiyuan ，China; 2. School of Forensic Medicine，Shanxi Medical University，Taiyuan ，China; 3. School of Basic Medical Science，Shanxi Medical University，Taiyuan ，China |
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| Abstract: |
| Isoniazid（INH） is an indispensable drug in the treatment of tuberculosis at this stage．The determination of INH content is of great significance in drug quality control and drug safety．In this paper，carbon dots（CDs） were synthesized rapidly by a microwave method using citric acid as the carbon source and thiourea as the nitrogen source．The carbon dots emit blue fluorescence under UV light，which have the advantage of biocompatibility and high stability．The carbon dots were mixed with manganese dioxide nanosheets（MnO2） to form a fluorescent nanoprobe for the sensitive detection of INH．The morphology，composition and surface groups of the carbon dots and manganese dioxide nanosheets were characterized by transmission electron microscopy（TEM），X ray photon spectroscopy（XPS），X ray diffraction（XRD），Fourier transform infrared spectroscopy（FT－IR），ultraviolet－visible spectroscopy（UV－Vis） and fluorescence spectroscopy，respectively．TEM results indicated that the carbon dots were nearly spherical with particle sizes of 2.0－3.0 nm，while the manganese dioxide nanosheets had a typical two-dimensional flake morphology．Fourier transmission infrared spectroscopy revealed that there were hydroxyl，amino，and carboxyl groups on the surface of carbon dots．The fluorescence spectrum of carbon dots demonstrated a maximum emission at 445 nm with an excitation wavelength of 360 nm．Meanwhile，manganese dioxide nanosheets had a wide absorption band of 250－600 nm，which had a large overlap with the fluorescence emission spectra of carbon dots．The fluorescence of carbon dots was quenched by fluorescence resonance energy transfer（FRET） between carbon dots and manganese dioxide nanosheets．After the addition of trace INH，the redox reaction between INH and manganese dioxide nanosheets occurred，which degraded manganese dioxide nanosheets and restored the fluorescence of carbon dots．This allowed the development of a nanoprobe for the quantitative detection of INH，which showed good sensitivity and selectivity for INH．The fluorescence intensity of carbon dots at 360 nm excitation under different pH of PBS solution，different concentration of sodium chloride solution and UV irradiation was studied．The experimental results showed that carbon dots had good stability．After optimization of the experimental conditions， the linear range of the nanoprobe for INH detection in pH 7.4 PBS buffer solution was 0.5－60 μmol/L，with a detection limit of 0.02 μmol/L．Selectivity and interference experiments presented that the nanoprobe was highly selective for INH．Moreover，common metal ions， amino acids，and sugars did not interfere with INH detection．The CDs/MnO2 probe was used for the determination of INH in normal human blood，urine samples，and tablets．The recoveries of blood samples，urine samples and tablets were 94.8%－116%，99.0%－105% and 96.8%－102%，respectively．Relative standard deviations（RSDs） were less than 5% for all samples．It is highly sensitive，selective，simple to prepare，economical，and environmentally friendly．The probe provides a new idea for the detection of INH and has a promising application prospect in the detection of biological samples． |
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