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| Determination of 8-Oxyguanine DNA Glycosylase Using a Fluorescence Sensor Based on PET Effect |
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| DOI： |
| KeyWord:8-oxyguanine DNA glycosylase photo-induced electron transfer fluorescence sensor |
| Author | Institution |
| LIU Ping，JIN Dong，LI Zhe-jian，QIAO Cheng-fang |
1. College of Chemical Engineering and Modern Materials，Shangluo University，Shangluo ，China; 2. Shaanxi Key Laboratory of Tailings Comprehensive Utilization of Resources，Shangluo ，China; 3. College of Material Science and Engineering，Nanjing University of Posts & Telecommunications， Nanjing ，China |
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| Abstract: |
| 8-Oxyguanine DNA glycosylase（OGG1） is an impotant DNA repaired enzyme，which can excise 8-oxyguanine（8-oxoG），a marker of damaged DNA．Meanwhile，it is implicated in the etiology of many kinds of cancers．Here，a fluorescence sensor based on the photo-induced electron transfer（PET） effect was constructed to detect the quantities of OGG1 which depended on the change of fluorescence intensity before and after addition of the OGG1 in the system．The carefully designed DNA signal probe contained an internal 8-oxoG site and 5' end carboxyfluorescein tag，a complementary sequence as the quenching group with 3' terminal containing multiple G base．This method was simple，sensitive and specific，and it was used for the detection of OGG1 in human serum samples．The results showed that there was a good linear relationship between the fluorescence intensity change of the system and OGG1 in the concentration range of 0－60 U/mL，with a detection limit of 0.7 U/mL．The recoveries for OGG1 in spiked human blood serum samples ranged from 99.1% to 105%，with the relative standard deviations（RSDs） of 1.0%－4.3%．The sensor has the advantages of good selectivity，simple operation and low cost． |
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