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| Advances on Single Particle Analysis for Extracellular Vesicles |
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| DOI： |
| KeyWord:extracellular vesicles（EVs） single particle analysis fluorescence microscopy flow cytometry nano-flow cytometry |
| Author | Institution |
| HU Yun-yun，DI Hao-nan，LAI Hui-yan，LI Yu-rou，CHEN Chen，TIAN Ye，YAN Xiao-mei |
Department of Chemical Biology，MOE Key Laboratory of Spectrochemical Analysis & Instrumentation， Key Laboratory for Chemical Biology of Fujian Province，College of Chemistry and Chemical Engineering，Xiamen University，Xiamen ，China |
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| Abstract: |
| Extracellular vesicles（EVs） are nanoscale membrane-bound vesicles secreted by almost all the cell types to mediate intracellular communication via transferring bioactive molecules including proteins，nucleic acids and lipids．EVs have stimulated considerable scientific and clinical interest owing to their active participations in multiple pathophysiological processes and potential utility as diagnostic markers and therapeutic entities．However，EVs are extremely heterogeneous not only in particle size but also in the molecular compositions．Therefore，there is a desperate need to develop advanced techniques facilitating quantitative multiparameter analysis of single EV to identify different EV subtypes，unraveling their biological functions，and promoting the development of EV－based clinical applications．In this review， the emerging techniques for single EV analysis（SVA） in recent years are summarized．The technological advances in the characterization and phenotyping of EVs are described．Moreover，new insights into the challenges and the future development of SVA are given. |
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