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| A Sandwich-type Electrochemical Immunosensor Based on Platinum Hybrid Multiwalled Carbon Nanotubes Adhered Copper Oxide |
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| DOI： |
| KeyWord:SnO2 loaded graphene sheet hybridized by Au NPs Pt hybrid multiwalled carbon nanotubes adhered copper oxide immunosensor hepatitis B surface antigen |
| Author | Institution |
| SUN Jing-bo，LUAN Ye-bo，MA Shi-hua，ZHENG Zhao，XING Yi-wen，ZHANG Hong-jing |
1. College of Engineering，Yantai Nanshan University，Yantai ，China;
2. College of Chemistry Engineering，Yantai Nanshan University，Yantai ，China;
3. Hangxin Material Technology Co.Ltd．，Yantai ，China |
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| Abstract: |
| A novel sandwich-type electrochemical immunosensor was designed for quantitative detection of hepatitis B surface（HBs） antigen．SnO2 loaded graphene sheet hybridized by Au NPs（GS-SnO2－Au） was used as the platform．Platinum hybrid multiwalled carbon nanotubes adhered copper oxide（Pt@CuO－MWCNTs） was used as label．As the carrier material，GS－SnO2－Au has larger surface area，better electrical conductivity，dispersibility and biological compatibility，which is benefit for capturing antibodies（Ab1）．Pt，CuO and multiwalled carbon nanotubes could be used as catalysts for the decomposition of hydrogen peroxide，and Pt@CuO－MWCNTs could realize multiple amplified signals due to their synergy．The morphological characteristics of GS－SnO2－Au and Pt@CuO－MWCNTs were observed by transmission electron microscopy（TEM）．The results showed that the substrate material（GS－SnO2－Au） and the marker material（Pt@CuO－MWCNTs） have excellent properties．The synthesis of Pt@CuO－MWCNTs was further characterized by energy spectrum，and the element content was also analyzed．The assembly process of the immunosensor was characterized by cyclic voltammetry（CV） and alternating current impedance．The optimal conditions were as following：pH value of phosphate buffer solution was 7.4，concentration of GS－SnO2－Au was 1 mg/mL，and concentration of Pt@CuO－MWCNTs was 2 mg/mL．Under the optimal conditions，the designed immunosensor exhibited a wide linear in the range of 0.001－100 ng/mL with a low detection limit of 0.33 pg/mL for HBs．The spiked recoveries for HBs in serum samples were in the range of 99.8%－100% with relative standard deviations（RSDs） of 1.1%－1.5%．With good selectivity，reproducibility and stability，the designed immunosensor could provide potential applications in clinical monitoring of HBs. |
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