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| Fabrication and Characterization of Monoclonal Antibody Against Isoprothiolane |
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| DOI： |
| KeyWord:isoprothiolane monoclonal antibody ELISA pesticide |
| Author | Institution |
| WANG Xing-xing，WANG Yao-yu，LI Xiao-na，SHEN Chao-qun，ZHOU Kai，ZHANG Jing |
1. Shenzhen Total-testing Technology Co.，Ltd.，Shenzhen ，China;
2. Institute of Jiangxi Oil-tea Camellia，Jiujiang University，Jiujiang ，China;
3. Technology Center of Yantai Customs，Yantai ，China |
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| Abstract: |
| An enzyme-linked immunosorbent assay（ELISA） method based on the monoclonal antibody was established for determination of pesticide isoprothiolane．Two haptens were prepared by extending active arms of different lengths from disulfide heterocyclic pentane structure of isoprothiolane molecule．The two haptens were conjugated with carrier protein to obtain the immunogen and coating antigen．Monoclonal antibody，named mAb－DWL，against isoprothiolane was obtained via the procedures of mouse immunization，cell fusion，eluting sieve and ascites collection．Results showed that the ELISA based on the obtained mAb－DWL exhibited a linear range of 4.6－530.2 ng/mL，with an IC50 of 55.2 ng/mL．Meanwhile，the cross-reactivity rates for the mAb－DWL with other similar pesticides were lower than 1%．The spiked recoveries for isoprothiolane in the vegetables and grains samples ranged from 77.2% to 116%．The monoclonal antibody prepared by this method could be used for development of rapid method for isoprothiolane determination. |
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