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| Screening of Illegally Added Chemicals in Traditional Chinese Veterinary Medicine Oral Liquid by High Resolution Mass Spectrometry |
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| DOI： |
| KeyWord:traditional Chinese veterinary medicine oral liquid illegally added high-resolution mass spectrometry high-throughput screening |
| Author | Institution |
| HUO Ning-ning，LI Yan-dong，HAN Xue，AI Lian-feng，WANG Zhen-lai |
1. College of Food and Biology，Hebei University of Science and Technology，Shijiazhuang ，China; 2. Hebei Provincial Station of Veterinary Drug and Feed，Shijiazhuang ，China; 3. Shijiazhuang Customs Technology Center，Shijiazhuang ，China |
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| Abstract: |
| A high-throughput screening method for 167 illegally added chemical drugs in traditional Chinese veterinary medicine oral liquid was established by ultra-high performance liquid chromatography－quadrupole electrostatic field orbit trap mass spectrometry．The samples were extracted with 0.5% formic acid acetonitrile－water by oscillation ultrasonic extraction，and the data were collected with electrospray ionization source（ESI） in positive and negative ion scanning mode．By comparing the self-built the basic information and MS information database of 167 compounds，the chromatographic conditions，MS conditions，extraction solvent types and purification conditions were optimized，and the test results were analyzed．The results showed that the oral liquid samples after pretreatment were tested on the machine，and the data results were analyzed by Trace Finder screening software．It was found that the concentrations of illegally added drugs could be qualitatively detected when their concentrations were 50 ng/mL（cephalosporin and lactam drugs were 100－200 ng/mL）．The detection rates for illegally added drugs were slightly different due to the influence of matrixes．With the characteristics of simple operation，rapidness and efficiency，this method could basically achieve the purpose of high-throughput rapid screening of illegally added chemical drugs in traditional Chinese veterinary medicine oral liquid，and it lays a foundation for the screening of illegally added chemical drugs in other veterinary drug formulations. |
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