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| Quality Evaluation of Tetrastigmae Radix under Different Drying Methods Based on Simultaneous Determination of Multiple Bioactive Constituents Combined with Multivariate Statistical Analysis |
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| DOI： |
| KeyWord:Tetrastigmae Radix different drying methods multiple active constituents multivariate statistical analysis ultra-fast liquid chromatography－triple quadrupole－linear ion trap mass spectrometry（UFLC－QTRAP－MS/MS） |
| Author | Institution |
| CHEN Hai-jie，ZHOU Yong-yi，XUE Jia，ZOU Li-si，WU Nan，YUAN Jia-huan，LIU Xun-hong，YANG Wei，CHENG Jian-ming |
1. School of Pharmacy，Nanjing University of Traditional Chinese Medicine，Nanjing ，China; 2. Engineering Research Center for Classical Recipes of Jiangsu Province，Nanjing ，China |
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| Abstract: |
| An ultra-fast liquid chromatography－triple quadrupole－linear ion trap mass spectrometry（UFLC－QTRAP－MS/MS） was developed for the rapid and simultaneous analysis of 60 active constituents including flavonoids，tannins，phenolic acids，nucleosides，polyphenols and amino acids in Tetrastigmae Radix，which was combined with multivariate statistical analysis to evaluate the quality of Tetrastigmae Radix dried or processed by different methods．The gradient separation was performed on an XBridge?C18 column（4.6 mm × 100 mm，3.5 μm） with 0.4% formic acid－methanol as mobile phase，and the mass spectrometric detection was carried out in electrospray positive or negative ion mode under multiple reaction monitoring（MRM） mode. The samples of differently dried and processed Tetrastigmae Radix were comprehensively evaluated by entropy weight TOPSIS，analysis of variance（ANOVA） method and gray correlation analysis（GRA） according to the contents of 60 target constituents．The results showed that there were good linear relationships for 60 target constituents in a certain mass concentration range，with their correlation coefficients（r2） not less than 0.999 0．The limits of detection（LODs） and the limits of quantitation（LOQs） were in the range of 0.03－13.59 ng/mL and 0.09－45.30 ng/mL，respectively．The average spiked recoveries for the samples ranged from 96.1% to 102%，with relative standard deviations（RSDs） less than 5%．Different drying methods have certain effects on the multiple active constituents in Tetrastigmae Radix，and modern drying methods could significantly retain the active constituents in Tetrastigmae Radix compared with the traditional drying methods．The ANOVA showed that the low temperature drying treatment had a good retention effect on flavonoids，tannins，and polyphenols．The loss of nucleosides and phenolic acids in Tetrastigmae Radix was low under vacuum drying conditions．Sun-drying or shade-drying treatment tends to cause the loss of amino acids in Tetrastigmae Radix．The results of GRA and TOPSIS analyses were the same，and the quality ranking of each group was the same，indicating that different drying methods had significant effects on the active constituents in Tetrastigmae Radix，with large differences between groups．GRA and TOPSIS analyses both indicated that the comprehensive quality of Tetrastigmae Radix samples treated by vacuum drying was better，while the overall quality of Tetrastigmae Radix treated by freeze-drying，hot-air drying，sun-drying，and shade-drying was relatively poor．TOPSIS was more suitable than GRA for distinguishing the quality of Tetrastigmae Radix samples．The comprehensive analysis showed that the comprehensive quality of the vacuum-drying treated Tetrastigmae Radix was better under the five drying and processing methods．The method developed in this study is accurate and stable，which could provide an experimental basis for the rational drying and processing of Tetrastigmae Radix，and also provide a new method reference for the comprehensive evaluation and overall control of the quality of Tetrastigmae Radix herb. |
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