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| Direct Electrochemical Behaviors of Glucose Oxidase on Hollow Porous Gold Nanoparticles/Reduced Graphene Oxide Nanocomposite and Its Biosensing Application |
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| DOI： |
| KeyWord:glucose oxidase hollow porous gold nanoparticles reduced graphene oxide electrochemical biosensor |
| Author | Institution |
| YIN Xue-hu，YANG Xin-jie，LUO Dan，ZHANG Yan-li，WANG Hong-bin，YANG Wen-rong，PANG Peng-fei |
1. Key Laboratory of Environmental Functional Materials of Yunnan Province Education Department，School of Chemistry and Environment，Yunnan Minzu University，Kunming ，China; 2. School of Life and Environmental Sciences，Deakin University，Geelong VIC 3217，Australia |
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| Abstract: |
| Hollow porous gold nanoparticles（HPAuNPs） were prepared by a simple solution phase sacrificial template method．The hollow porous gold nanoparticles/reduced graphene oxide（HPAuNPs/rGO） nanocomposite was prepared for the effective immobilization of glucose oxidase（GOx） on the surface of glassy carbon electrode（GCE）．An electrochemical biosensor for detection of glucose was constructed based on GOx/HPAuNPs/rGO modified GCE．Scanning and transmission electron microscopy，X-ray photoelectron spectroscopy，X-ray diffraction spectroscopy，infrared spectroscopy and electrochemistry were used to characterize the morphology and structure of the nanomaterial，the immobilization process of GOx，and the direct electrochemical behaviors and the electrocatalytic properties of the biosensor．The results showed that the HPAuNPs/rGO nanocomposite could effectively accelerate the direct electron transfer（DET） between GOx and electrode due to the synergy effect of HPAuNPs and rGO．The modified electrode exhibited an excellent electrocatalytic property for glucose determination．The response current was proportional to the concentration of glucose in the range of 0.05－7.0 mmol/L，with a detection limit（S/N = 3） of 16 μmol/L．With good selectivity，reproducibility and stability，the proposed biosensor could satisfy the demands for glucose detection in real serum sample，with recoveries of 98.0%－103%，and relative standard deviations（RSDs） not more than 5.0%. |
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