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| Determination of Ethoxyquinoline and Ethoxyquinoline Dimer in Aquatic Products by High Performance Liquid Chromatography－Tandem Mass Spectrometry |
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| DOI： |
| KeyWord:high performance liquid chromatography－tandem mass spectrometry aquatic products ethoxyquinoline ethoxyquinoline dimer solid-phase extraction |
| Author | Institution |
| WANG Meng-ru，YIN Yao，NING Xiao-pan，ZHANG Ping，PENG Jun，LOU Yun，DING Tao，WU Li-na，LIU Han，ZHANG Xiao-yan |
1. Animal，Plant and Food Inspection Center，Nanjing Customs，Nanjing ，China; 2. College of Food and Pharmaceutical Engineering，Nanjing Normal University，Nanjing ，China |
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| Abstract: |
| An analytical method was developed for the simultaneous determination of ethoxyquinoline and ethoxyquinoline dimer residues in aquatic products such as fishes，shrimps，loaches and crabs by high performance liquid chromatography－tandem mass spectrometry（HPLC－MS/MS）．An appropriate amount of vitamin C was added in the sample preparation．Acetonitrile was used as the extraction solvent，and hexane was used for liquid－liquid extraction．The supernatant was cleaned up on an Oasis PRiME HLB solid-phase extraction column and spun to near dryness，acetonitrile was used to fix the volume．Then the sample was separated on an Agela Phenomenex Kinetex C18 column（100 mm ×4.6 mm × 2.6 μm），and subsequently detected with electrospray ionization source in positive ionization mode under selected reaction monitoring（SRM） mode，and quantified by external standard method. There were good linear relationships for both ethoxyquinoline and ethoxyquinoline dimer in concentration range of 1－100 μg/L，with their correlation coefficients（r2） greater than 0.994. The limits of detection（LODs） and limits of quantitation（LOQs） of the method were 0.5 μg/kg and 1.5 μg/kg，respectively. The average recoveries for two target substances at four spiked levels of 1.5，5，10 and 100 μg/kg ranged from 75.8% to 103%，with relative standard deviations（RSDs） of 0.58%－9.6%. The developed method is sensitive，accurate and precise，and it could be applied to the determination of ethoxyquinoline and ethoxyquinoline dimer in aquatic products such as fishes，shrimps，loaches and crabs. |
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