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| Analysis of Chemical Constituents in Fuke Zaizao Pill by Ultra Fast Liquid Chromatography－Ion Trap Time-of-flight Mass Spectrometry |
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| DOI： |
| KeyWord:Fuke Zaizao pill ultra fast liquid chromatography－ion trap－time-of-flight mass spectrometry（UFLC－IT－TOF MS） chemical substance basis quality evaluation secondary development |
| Author | Institution |
| ZHOU Xue，GE Qiu-ping，PENG Xiao-yan，WANG Xue-li，SHANG Mi，MAO Song，YU Yuan，GE Fa-huan |
1. School of Pharmaceutical Sciences，Sun Yat-sen University，Guangzhou ，China; 2. Guiyang Dechangxiang Pharmaceutical Co.，Ltd.，Guiyang ，China; 3. School of Traditional Chinese Medicine，Guangdong Pharmaceutical University，Guangzhou ，China; 4. Nansha Research Institute，Sun Yat-sen University，Guangzhou ，China |
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| Abstract: |
| Fuke Zaizao pill consists of forty-two herbs，which is commonly utilized in clinic therapy for gynecological diseases．However，Fuke Zaizao pill has complicated chemical compositions that have not been adequately clarified，and its quality control needs to be improved．In this paper，an ultra fast liquid chromatography－ion trap time-of-flight mass spectrometry（UFLC－IT－TOF MS） was developed for rapid analysis of the chemical constituents in Fuke Zaizao pill．The gradient elution was performed on a COSMOSIL C18 column（3.0 mm × 150 mm，2.6 μm） with 0.4% acetic acid and acetonitrile as mobile phases．Positive and negative ion ESI modes were used simultaneously to collect MS1，MS2 and MS3 fragment information of Fuke Zaizao pill．A total of 155 chemical constituents were tentatively identified by deducing the mass spectrometric cleavage pattern，and comparing with standards，references and single herb chromatography，including 47 terpenoids，43 phenols and phenolic acids，39 flavonoids，14 alkaloids，8 phthalides，and 4 other structures，of which 133 components were firstly found in the Fuke Zaizao pill．The chemical composition of Fuke Zaizao pill was further elucidated in this paper，and a wealth of information for improving its quality standards and elucidating its effective components was provided. |
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