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| Quality Difference Analysis of Salviae Miltiorrhizae Radix et Rhizoma from Different Origins Based on Multi-index Content Determination Combined with Chemometrics |
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| DOI： |
| KeyWord:Salviae Miltiorrhizae Radix et Rhizoma origin difference content determination chemometrics quality evaluation |
| Author | Institution |
| WANG Qiao，YU Yong-jie，FU Hai-yan，LI Xiang，YANG Jian |
1. College of Pharmacy，Nanjing University of Chinese Medicine，Nanjing ，China; 2. State Key Laboratory Breeding Base of Dao-di Herbs，Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing ，China; 3. College of Pharmacy，Ningxia Medical University，Yinchuan ，China; 4. School of Pharmaceutical Sciences， South-Central University for Nationalities，Wuhan ，China |
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| Abstract: |
| Ultra-performance liquid chromatography（UPLC） and ultra-performance liquid chromatography－triple quadrupole mass spectrometry（UPLC－QQQ－MS） were adopted for the simultaneous determination on 23 chemical ingredients in a total of 408 Salviae Miltiorrhizae Radix et Rhizoma（Danshen） samples from Shandong，Henan，Shaaxi，Sichuan and Anhui provinces，in order to compare and analyze the influences of origins on phenolic acids and tanshinones in Danshen．Meanwhile，the data were analyzed by multivariate statistics．The results showed that 17 phenolic acids and 6 tanshinones had significant differences in Danshen．The samples from Shandong province had the highest content of tanshinones，while the samples from Sichuan province had the highest content of salvianolic acid B，and the samples from Anhui province had the highest contents of lithospermic acid，salvianolic acid Y，salvianolic acid A，salvianolic acid D and salvianolic acid E．Various pattern recognition methods were used to identify and analyze Danshen from different origins，and linear discriminant analysis（LDA） was the best model for traceability．Orthogonal partial least squares－discriminant analysis（OPLS－DA） showed that the chemical constituents of Danshen from different origin areas were significantly different．Quality markers of Danshen from different sources were not limited to salvianolic acid B，tanshinone Ⅰ，cryptotanshinone and tanshinone ⅡA，other salvianolic acids and tanshinones were also important quality markers. In this paper，the multi-component content determination and data analysis of cultivated medicinal materials collected from different main origin areas of the country were carried out．The quantitative method established was highly specific，accurate and efficient，which provided a reference for the quality control and identification of Danshen from different producing areas. |
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